單色顯微鏡數(shù)碼相機(jī) DS-Qi2

弱熒光拍攝與寬視野

DS-Qi2單色相機(jī)具備高像素密度、高靈敏度與低噪聲的特性。

全畫幅CMOS傳感器

繼承自專業(yè)數(shù)碼單反相機(jī)并由尼康制造的CMOS傳感器和成像技術(shù)，經(jīng)過優(yōu)化現(xiàn)已用于顯微技術(shù)。

高靈敏度

----可檢測(cè)微弱熒光信號(hào)

7.3um像素尺寸，高量子效率配合低讀出噪聲使DS-Qi2甚至可以檢測(cè)微弱的熒光信號(hào)。

Indian Muntjac Deer Skin Fibroblast Cells, Cytoskeletal F-actin labeled with Alexa Fluor 488

Sample courtesy of: Michael Davidson and Florida State University

的線性特性

----可實(shí)現(xiàn)可靠的定量分析

±1%的線性誤差，DS-Qi2是熒光強(qiáng)度測(cè)量的絕好工具。無論時(shí)間強(qiáng)度測(cè)量還是比率測(cè)量都可輕松應(yīng)對(duì)。

高幀率

----即使使用熒光圖像亦可實(shí)現(xiàn)快速對(duì)焦

2.2個(gè)電子讀出噪聲配合大容量像素及0.6個(gè)電子暗電流使其可以拍攝極小噪聲的熒光圖像。

低噪聲

----獲取微弱熒光的極低噪聲圖像

2.2個(gè)電子讀出噪聲配合大容量像素，使獲取的熒光圖像具有極低的噪聲和的動(dòng)態(tài)范圍。

LLC-PK1 cells expressing GFP-EB3 tubulin Low noise and large linear full well capacity allows the acquisition of large dynamic range in a single capture.
A captured image is n intensity scaled to both the brightest and dimmest areas.
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

時(shí)間序列成像

----使用NIS-Elements軟件進(jìn)行熒光時(shí)間序列成像

結(jié)合寬視野和高像素密度及低噪聲，DS-Qi2是時(shí)間序列拍攝應(yīng)用的選擇。

Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the timelapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path.
DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped timelapse sequence from a large FOV image.
Objective: CFI Plan Apochromat λ 60x oil / NA: 1.4)
Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University

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