mRNA的瞬轉(zhuǎn)試劑，能在15-30后檢測到蛋白質(zhì)合成
高轉(zhuǎn)染效率，針對原代細(xì)胞（例如，HUVEC和神經(jīng)元）和干細(xì)胞的轉(zhuǎn)染試劑
快速mRNA導(dǎo)入技術(shù)，無胞吞作用及溶酶體的降解影響，不需要將基因?qū)爰?xì)胞核內(nèi)
低的細(xì)胞毒性
mRNA翻譯和降級(jí)研究
蛋白質(zhì)生化研究：合成，折疊，處理，穩(wěn)定性，定位，降解
mRNA轉(zhuǎn)染進(jìn)入原代細(xì)胞中不需要基因修飾的組織
采用RNA-only CRISPR/Cas技術(shù)進(jìn)行基因組改造工程
全新的mRNA活細(xì)胞轉(zhuǎn)染試劑
15-30分鐘能完成mRNA轉(zhuǎn)染
不需要胞吞過程
不需要溶酶體降解
不需要將基因轉(zhuǎn)入細(xì)胞核中
專為成熟的（帶5’-Capped和poly-A的）mRNA優(yōu)化的轉(zhuǎn)染試劑
較好的生物兼容性和極低的生物毒性
不像傳統(tǒng)的脂質(zhì)體轉(zhuǎn)染技術(shù)，膜融合不需要胞吞作用來釋放mRNA。內(nèi)含mRNA的Fuse-it的脂載體，能夠快速簡單的與細(xì)胞膜相融合，并且將mRNA直接釋放如細(xì)胞質(zhì)中。mRNA翻譯工作能夠立即進(jìn)行，不會(huì)受到胞吞，溶酶體降解，或者有絲分裂的影響。
| | |
 | Fuse-it-mRNA具有令人難以置信的極速mRNA表達(dá)。在CHO-K1細(xì)胞中6個(gè)小時(shí)就具有接近99%的表達(dá)效率。而傳統(tǒng)的DNA轉(zhuǎn)染技術(shù)的效率低于10%。 |
| | |
Fuse-it-RNA采用與細(xì)胞膜一致的電極，能夠有效的和多種細(xì)胞系進(jìn)行膜融合。細(xì)胞系，無神經(jīng)元，各種難以轉(zhuǎn)染的原代細(xì)胞都能采用膜融合轉(zhuǎn)染試劑將mRNA轉(zhuǎn)入細(xì)胞質(zhì)中。Fuse-it-mRNA的膜融合技術(shù)能夠獲得快速高效的蛋白表達(dá)而不會(huì)對基因組造成影響。
Fuse-it-mRNA操作步驟非常容易掌握，因?yàn)槿诤显噭┖蚼RNA的比例在各個(gè)實(shí)驗(yàn)中都是固定的。試劑安全性好，沒有任何基因毒性，操作不需進(jìn)行嚴(yán)格的生物防護(hù)。
對比傳統(tǒng)的脂質(zhì)體轉(zhuǎn)染試劑，F(xiàn)use-It-mRNA僅僅需要5-20分鐘的孵育時(shí)間就能達(dá)到較大的mRNA轉(zhuǎn)染效率。不僅如此，僅用很少量的Fuse-it試劑就能獲得較大量的mRNA的轉(zhuǎn)染效率，而不需要額外使用化學(xué)藥劑，這樣對于一些敏感的細(xì)胞不會(huì)造成細(xì)胞毒性。
 | 對比于傳統(tǒng)的脂質(zhì)體轉(zhuǎn)染技術(shù)，F(xiàn)use-It-mRNA的膜融合方式不會(huì)傷害到細(xì)胞。如圖，六小時(shí)后，采用Fuse-It-mRNA轉(zhuǎn)染的人正常表皮角細(xì)胞（nHEK）已有 表達(dá)，而且細(xì)胞活性。而采用傳統(tǒng)方法時(shí)，六個(gè)小時(shí)后大量的細(xì)胞都已經(jīng)死亡
| |
2小時(shí)內(nèi)就能在細(xì)胞中檢測到eGFP的蛋白表達(dá)，并且，eGFP表達(dá)量在持續(xù)上升并且能持續(xù)穩(wěn)定表達(dá)3天以上（90個(gè)小時(shí)）。
注意：采用成熟的mRNA能獲得理想的實(shí)驗(yàn)結(jié)果（帶5’Capped和3’Poly-A的mRNA）.
| | | | |
| 細(xì)胞類型 | 組織 | 孵育時(shí)間 | mRNA表達(dá)效率 |
| Primary Cells |
| Bone Marrow-derived Dendritic Cells (BMDCs) | murine | 20 min | 70-80% |
| Cortical Neurons (embry.) | rat | 5-15 min | 70-90% |
| Fibroblasts, foreskin | human | 10-15 min | 60-80% |
| Hepatocytes | human | 15-25 min | 60-80% |
| HUVEC | human | 10-20 min | 50-70% |
| Macrophages/Monocytes | human | 10-15 min | 30-50% |
| Myocytes (embry.) | rat | 15-25 min | 40-60% |
| Myofibroblasts (embry.) | rat | 15-20 min | 30-50% |
| N/TERT-1 (keratinocytes, immortalized by expression of TERT) | human | 10 min | 80-90% |
| nHEK, foreskin | human | 5-15 min | 70-80% |
| ZNS cells (unpurified) | rat | 5-15 min | 80-99% |
| Stem Cells |
| Cor.4U Myocytes (iPSC derived cardiomyocytes) | human | 10-20 min | 80-99% |
| Dopa.4U (iPSC derived dopaminergic neurons) | human | 5-10 min | 70-90% |
| iPSC | human | 10-20 min | 70-90% |
| Peri.4U (iPSC derived peripheral neurons) | human | 5-10 min | 70-90% |
| Cell Lines |
| A549 | human | 20-25 min | 80-99% |
| CHO-K1 | hamster | 15-20 min | 80-99% |
| HeLa | human | 15-20 min | 70-90% |
HL-1
| mouse | 15-20 min | 5-10% |
| HT-1080 | human | 15-20 min | 80-99% |
| HUH-7 | human | 10-15 min | 70-90% |
| Jurkat | human | 1-2 min | 20-50% |
| L929 | mouse | 15-20 min | 70-90% |
| MCF-10A | human | 10-15 min | 80-99% |
| MCF-7 | human | 15-20 min | 70-90% |
| MDA-MB-231 | human | 10-15 min | 70-90% |
| MDCK | dog | 20-25 min | 20-40% |
| NIH-3T3 | mouse | 15-20 min | 70-90% |
| Rat1 | rat | 5-10 min | 80-99% |
| RAW 264.7 | rat | 15-20 min | 50-70% |
| THP-1 | human | 1-2 min | 40-70% |
| |
| | | | | | |  | Cortical Neurons (embry.) | | Organism: | rat | | Incubation Time: | 15 min | | mRNA | eGFP | |  | Fibroblasts, foreskin | | Organism: | human | | Incubation Time: | 15 min | | mRNA | eGFP | |  | Hepatocytes | | Organism: | human | | Incubation Time: | 25 min | | mRNA | LifeAct-TagGFP2 | |  | THP-1 | | Organism: | human | | Incubation Time: | 1-2 min | | mRNA | eGFP | | | | | | | | | | | | | |  | HUVEC | | Organism: | human | | Incubation Time: | 20 min | | mRNA | eGFP | | | | | | |  | HUVEC | | Organism: | human | | Incubation Time: | 20 min | | mRNA | LifeAct-TagGFP2 | |  | Myocytes (embry.) | | Organism: | rat | | Incubation Time: | 25 min | | mRNA | eGFP | |  | Myofibroblasts (embry.) | | Organism: | rat | | Incubation Time: | 20 min | | mRNA | eGFP | | | |  N/TERT-1
(keratinocytes, immortalized by expression of TERT) | | Organism: | human | | Incubation Time: | 10 min | | mRNA | eGFP | | |  | ZNS cells (unpurified) | | Organism: | rat | | Incubation Time: | 15 min | | mRNA | eGFP | | | |  | nHEK, foreskin | | Organism: | human | | Incubation Time: | 20 min | | mRNA | eGFP | |  | nHEK, foreskin | | Organism: | human | | Incubation Time: | 20 min | | mRNA | LifeAct-TagGFP2 | | | | | | | | | | | | |  | Cor.4U Myocytes | | Organism: | human | | Incubation Time: | 20 min | | mRNA | eGFP | |  | Cor.4U Myocytes | | Organism: | human | | Incubation Time: | 20 min | | mRNA | LifeAct-TagGFP2 | |  | Dopa.4U | | Organism: | human | | Incubation Time: | 10 min | | mRNA | eGFP | |  | iPSC | | Organism: | human | | Incubation Time: | 20 min | | mRNA | eGFP | | | |  | Peri.4U | | Organism: | human | | Incubation Time: | 10 min | | mRNA | eGFP | | | | | | | | | | | | | CHO-K1 | | Organism: | hamster | | Incubation Time: | 20 min | | mRNA | eGFP | |  | CHO-K1 | | Organism: | hamster | | Incubation Time: | 20 min | | mRNA | mCherry | |  | HeLa | | Organism: | human | | Incubation Time: | 20 min | | mRNA | eGFP | |  | |
