PlantScreen植物表型成像系統(tǒng)由捷克PSI公司研制生產(chǎn),整合了Robotic自動化控制系統(tǒng)、智能LEDs光照培養(yǎng)系統(tǒng)、葉綠素熒光成像測量分析、植物熱成像分析、植物近紅外成像分析、植物高光譜分析、自動條碼識別管理、RGB真彩成像等多項技術(shù),以化的方式實現(xiàn)大量植物樣品——從擬南芥、玉米到各種其它植物的生理生態(tài)與形態(tài)結(jié)構(gòu)成像分析,用于高通量植物表型成像分析測量、植物脅迫響應(yīng)成像分析測量、植物生長分析測量、生態(tài)毒理學研究、性狀識別及植物生理生態(tài)分析研究等。XYZ三維移動成像版PlantScreen的優(yōu)勢在于可以在一套系統(tǒng)中同時測量高度差異較大的不同植物樣品,比如同時培養(yǎng)測量擬南芥和玉米。
PlantScreen系統(tǒng)包括如下成像分析功能:
1. 大型葉綠素熒光成像分析:單幅成像面積35x35cm,成像測量參數(shù)包括Fo, Fm, Fv, Fo’, Fm’, Fv’, Ft, Fv/Fm, Fv’/Fm’, Phi_PSII, NPQ, qN, qP, Rfd等幾十個葉綠素熒光參數(shù)
2. RGB成像分析:成像測量參數(shù)包括:
1) 葉面積(Leaf Area: Useful for monitoring growth rate)
2) 植物緊實度/緊密度(Solidity/Compactness. Ratio between the area covered by the plant’s convex hull and the area covered by the actual plant)
3) 葉片周長(Leaf Perimeter: Particularly useful for the basic leaf shape and width evaluation (combined with leaf area))
4) 偏心率(Eccentricity: Plant shape estimation, scalar number, eccentricity of the ellipse with same second moments as the plant (0...circle, 1...line segment))
5) 葉圓度(Roundness: Based on evaluating the ratio between leaf area and perimeter. Gives information about leaf roundness)
6) 葉寬指數(shù)(Medium Leaf Width Index: Leaf area proportional to the plant skeleton (i.e. reduction of the leaf to line segment))
7) 葉片細長度SOL (Slenderness of Leaves)
8) 植物圓直徑(Circle Diameter. Diameter of a circle with the same area as the plant)
9) 凸包面積(Convex Hull Area. Useful for compactness evaluation)
10) 植物質(zhì)心(Centroid. Center of the plant mass position (particularly useful for the eccentricity evaluation))
11) 節(jié)間距(Internodal Distances)
12) 生長高度(Growth Height)
13) 植物三維高度和寬度(Maximum Height and Width of Plant in 3 Dimensions)
14) 相對生長速率(Relative growth rate)
15) 葉傾角(Leaf Angle)
16) 節(jié)葉片數(shù)量(Leaf Number at Nodes)
17) 其它參數(shù)如用于植物適合度估算的顏色定量分級、綠度指數(shù)(Other parameters such as color segmentation for plant fitness evaluation, greening index and others)
3. 高光譜成像分析(選配),可成像并分析如下參數(shù):
1) 歸一化指數(shù)(Normalized Difference Vegetation Index (NDVI))
2) 簡單比值指數(shù)(Simple Ratio Index, Equation: SR = RNIR / RRED)
3) 改進的葉綠素吸收反射指數(shù)(Modified Chlorophyll Absorption in Reflectance Index (MCARI1), Equation: MCARI1 = 1.2 * [2.5 * (R790- R670) - 1.3 * (R790- R550)])
4) 化土壤調(diào)整植被指數(shù)(Optimized Soil-Adjusted Vegetation Index (OSAVI), Equation: OSAVI = (1 + 0.16) * (R790- R670) / (R790- R670 + 0.16))
5) 綠度指數(shù)(Greenness Index (G), Equation: G = R554 / R677)
6) 改進的葉綠素吸收反射指數(shù)(Modified Chlorophyll Absorption in Reflectance Index (MCARI), Equation: MCARI = [(R700- R670) - 0.2 * (R700- R550)] * (R700/ R670))
7) 轉(zhuǎn)換類胡羅卜素指數(shù)(Transformed CAR Index (TCARI), Equation: TSARI = 3 * [(R700- R670) - 0.2 * (R700- R550) * (R700/ R670)])
8) 三角植被指數(shù)(Triangular Vegetation Index (TVI), Equation: TVI = 0.5 * [120 * (R750- R550) - 200 * (R670- R550)])
9) ZMI指數(shù)(Zarco-Tejada & Miller Index (ZMI), Equation: ZMI = R750 / R710)
10) 簡單比值色素指數(shù)(Simple Ratio Pigment Index (SRPI), Equation: SRPI = R430 / R680)
11) 歸一化脫鎂作用指數(shù)(Normalized Phaeophytinization Index (NPQI), Equation: NPQI = (R415- R435) / (R415+ R435))
12) 光化學植被反射指數(shù)(Photochemical Reflectance Index (PRI), Equation: PRI = (R531- R570) / (R531+ R570))
13) 歸一化葉綠素指數(shù)(Normalized Pigment Chlorophyll Index (NPCI), NPCI = (R680- R430) / (R680+ R430))
14) Carter指數(shù)(Carter Indices?, Equation: Ctr1 = R695 / R420; Ctr2 = R695 / R760)
15) Lichtenthaler指數(shù)(Lichtenthaler Indices, Equation: Lic1 = (R790 - R680) / (R790 + R680); Lic2 = R440 / R690)
16) SIPI指數(shù)(Structure Intensive Pigment Index (SIPI), Equation: SIPI = (R790- R450) / (R790+ R650))
17) Gitelson-Merzlyak指數(shù) (Gitelson and Merzlyak Indices, Equation: GM1 = R750/ R550; GM2 = R750/ R700)
4. 熱成像分析(選配):用于成像分析植物在光輻射情況下的二維發(fā)熱分布,良好的散熱可以使植物耐受較長時間的高光輻射或低水條件(干旱)
5. 近紅外成像分析(選配):用于觀測分析植物的水分狀態(tài)及其在不同組織間的分布變異,處于良好澆灌狀態(tài)的植物表現(xiàn)出對近紅外光譜的高吸收性,而處于干旱狀態(tài)的植物則表現(xiàn)出對近紅外光譜的高反射性,通過分析軟件可以監(jiān)測分析從干旱脅迫到再澆灌過程中的整個過程動態(tài)及植物對干旱脅迫的響應(yīng)和水分利用效率,并形成假彩圖像,可以與植物的形態(tài)指數(shù)及葉綠素熒光指數(shù)進行相關(guān)分析研究。
系統(tǒng)配置與工作原理:
PlantScreen植物表型成像分析系統(tǒng)XYZ三維移動成像版(PlantScreen XYZ Rototic System)基本配置包括自動化XYZ三維操作系統(tǒng)、RGB成像、FluorCam葉綠素熒光成像等,可選配高光譜成像、植物熱成像、植物近紅外成像、植物標識系統(tǒng)等,成像系統(tǒng)由自動化三維機械臂移動到植物上方進行成像分析等。整套系統(tǒng)可根據(jù)用戶實際需要定制,高度契合用戶的實際需求。